Pharmaceutical Biotechnology - Faculty for Chemistry and Pharmacy

Publications Prof DI Dr Ernst Wagner (1985-1999)

  • Noe, C.R., Knollmüller, M., Wagner, E., und Völlenkle, H. (1985). Selektivitäten bei Acetalisierungsreaktionen enantiomerenreiner Lactole am Beispiel von Octahydro-8,9,9-trimethyl-5,8-methano-2H-1-benzopyran-2-ol. Chem.Ber. 118, 1733-1745.
  • Noe, C.R., Knollmüller, M., Wagner, E., und Völlenkle, H. (1985). Kohlenhydrat-Modelle, I. Kinetische und thermodynamische Effekte bei Acetalisierungsreaktionen enantiomerenreiner Thiolactole. Chem.Ber. 118, 3299-3310.
  • Noe, C.R., Knollmüller, M., und Wagner, E. (1986). Ein einfaches Verfahren zur Herstellung anellierter Thiophene. Monatsh. Chemie 117, 621-629.
  • Noe, C.R., Knollmüller, M., Oberhauser, B., Steinbauer, G., und Wagner, E. (1986). Chirale Lactole, VI. Eine Methode zur Bestimmung der Absolutkonfiguration chiraler alpha-hydroxysubstituierter Nitrile, Alkine und Aldehyde. Chem.Ber. 119, 729-743.
  • Wagner, E., Xiang, Y.-B., Baumann, K., Gück, J., und Eschenmoser, A. (1990). Chemie von alpha-Aminonitrilen. Aziridin-2-carbonitril, ein Vorläufer von rac-O3-Phosphoserinnitril und Glycolaldehyd-phosphat. Helv.Chim.Acta 73, 1391-1409.
  • Müller, D., Pitsch, S., Kittaka, A., Wagner, E., Wintner, C.E., und Eschenmoser, A. (1990) Chemie von alpha-Aminonitrilen. Aldomerisierung von Glycolaldehyd-phosphat zu racemischen Hexose-2,4,6-triphosphaten und (in Gegenwart von Formaldehyd) racemischen Pentose-2,4-diphosphaten: rac-Allose-2,4,6-triphosphat und rac-Ribose-2,4-diphosphat sind die Reaktionshauptprodukte. Helv.Chim.Acta 73, 1410-1468.
  • Wagner, E., Zenke, M., Cotten, M., Beug, H. and Birnstiel, M.L. (1990). Transferrin-polycation conjugates as carriers for DNA uptake into cells. Proc. Natl. Acad. Sci. USA 87, 3410-3414.
  • Zenke, M., Steinlein, P., Wagner, E., Cotten, M., Beug, H. and Birnstiel, M.L. (1990) Receptor-mediated endocytosis of transferrin polycation conjugates: An efficient way to introduce DNA into hematopoietic cells. Proc. Natl. Acad. Sci. USA 87, 3655-3659.
  • Cotten, M., Laengle-Rouault, F., Kirlappos, H., Wagner, E., Mechtler, K., Zenke, M., Beug, H. and Birnstiel, M.L. (1990) Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels. Proc. Natl. Acad. Sci. USA 87, 4033-4037.
  • Wagner, E., Cotten, M., Foisner, R. and Birnstiel, M.L. (1991). Transferrin-polycation-DNA complexes: The effect of polycations on the structure of the complex and DNA delivery to cells. Proc. Natl. Acad. Sci. USA 88, 4255-4259.
  • Wagner, E., Cotten, M., Mechtler, K., Kirlappos, H. and Birnstiel, M.L. (1991). DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety. Bioconjugate Chem. 2, 226-231.
  • Curiel, D.T., Agarwal, S., Wagner, E., and Cotten, M. (1991). Adenovirus enhancement of transferrin-polylysine-mediated gene delivery. Proc. Natl. Acad. Sci. USA 88, 8850-8854.
  • Cotten, M., Oberhauser, B., Brunar, H., Holzner, A., Issakides, G., Noe, C.R., Schaffner, G., Wagner, E., and Birnstiel, M.L. (1991). 2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event. Nucleic Acids Res. 19, 2629-2635.
  • Wagner, E., Oberhauser, B., Holzner, A., Brunar, H., Issakides, G., Schaffner, G., Cotten, M., Knollmüller, M., and Noe, C.R. (1991). A simple procedure for the preparation of protected 2'-O-methyl or 2'-O-ethyl ribonucleoside-3'-O-phosphoramidites. Nucleic Acids Res. 19, 5965-5971.
  • Noe, C.R., Knollmüller, M., Steinbauer G., Wagner, E., Kurner, H., Ettmayer, P., and Vollenkle H. (1991) A simple method to direct the reduction of alpha-alkoxy-carbonyl compounds. Monatshefte Chemie 122, 299-317.
  • Oberhauser, B., and Wagner, E. (1992). Effective incorporation of 2'-O-methyl-oligoribonucleotides into liposomes and enhanced cell association through modification with thiocholesterol. Nucleic Acids Res. 20, 533-538.
  • Curiel, D.T., Wagner, E., Cotten, M., Birnstiel, M.L., Agarwal, S., Li, C.-M., Loechel, S., and Hu, P.-C. (1992) High-efficiency gene transfer by adenovirus coupled to DNA-polylysine complexes. Human Gene Therapy 3, 147-154.
  • Curiel, D.T., Agarwal, S., Romer, N., Wagner, E., Cotten, M., Birnstiel, M.L., and Boucher, R.C. (1992). Gene transfer to respiratory epithelial cells via the receptor-mediated endocytosis pathway. Am. J. Resp. Cell Mol. Biol. 6, 247-252.
  • Wagner, E., Cotten, M., Zatloukal, K. and Birnstiel, M. (1992) Transferrin receptor mediated gene transfer, Quarterly News 1, 13-25.
  • Wagner, E., Zatloukal, K., Cotten, M., Kirlappos, H., Mechtler, K., Curiel, D.T., and Birnstiel, M.L. (1992). Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. Proc. Natl. Acad. Sci. USA 89, 6099-6103.
  • Cotten, M., Wagner, E., Zatloukal, K., Phillips, S., Curiel,D.T., and Birnstiel, M.L. (1992). High-efficiency receptor-mediated delivery of small and large (48kb) gene constructs using the endosome disruption activity of defective or chemically inactivated adenovirus particles. Proc. Natl. Acad. Sci. USA 89, 6094-6098.
  • Wagner, E., Plank, C., Zatloukal, K., Cotten, M., and Birnstiel, M.L. (1992) Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine/DNA complexes: Towards a synthetic virus-like gene transfer vehicle. Proc. Natl. Acad. Sci. USA 89, 7934-7938.
  • Zatloukal, K., Wagner, E., Cotten, M., Phillips, S., Plank, C., Steinlein, P., Curiel, D., and Birnstiel, M.L. (1992). Transferrinfection: A highly efficient way to express gene constructs in eukariotic cells. Ann. N. Y. Acad. Sci. 660, 136-153.
  • Plank, C., Zatloukal, K., Cotten, M., Mechtler, K., and Wagner, E. (1992) Gene transfer into hepatocytes using asialoglycoprotein receptor mediated endocytosis of DNA complexed with an artificial tetra-antennary galactose ligand. Bioconjugate Chem.3, 533-539.
  • Cotten, M., Wagner, E., and Birnstiel, M.L. (1993). Receptor mediated transport of DNA into eukariotic cells. Methods Enzymol. 217, 618-644.
  • Gao, L., Wagner, E., Cotten, M., Agarwal, S., Harris, C., Romer, M., Miller, L., Hu, P-c., and Curiel, D. (1993) Direct in vivo gene transfer to the airway epithelium employing adenovirus-polylysine-DNA complexes. Human Gene Therapy 4, 17-24.
  • Michael, S.I., Huang, C.-h., Romer, M.U., Wagner, E., Hu, P.-c. and Curiel, D.T. (1993) Binding-incompetent adenovirus facilitates molecular conjugate-mediated gene transfer by the receptor-mediated endocytosis pathway. J. Biol. Chem. 268, 6866-6869.
  • Cotten, M., Wagner, E., Zatloukal, K. and Birnstiel, M.L. (1993) Chicken adenovirus (CELO virus) particles augment receptor-mediated DNA delivery to mammalian cells and yield exceptional levels of stable transformants. J. Virol. 67, 3777-3785.
  • Wagner, E. (1993) Gentherapie: Neue Wege in der Krebsbehandlung. In "Genetische Kunst-Künstliches Leben", Ars Electronica 93, K. Gerbel, P. Weibel (Eds.) PVS Verleger, Wien, pp. 151-154.
  • Cotten, M. and Wagner, E. (1993) Non-viral approaches to gene therapy. Current Op. Biotech. 4, 705-710.
  • Zatloukal, K., Schmidt, W., Cotten, M., Wagner, E., Stingl, G., and Birnstiel, M.L. (1993) Somatic gene therapy for cancer: the utility of transferrinfection in generating ‘tumor vaccines‘. Gene 135, 199-207.
  • Harris, C.E., Agarwal S., Hu, P., Wagner E., and Curiel, D.T. (1993) Receptor-mediated gene transfer to airway epithelial cells in primary culture. Am. J. Respir. Cell Mol. Biol. 9, 441-7.
  • Noe, C.R., Knollmüller, M., Miculka, C., Dungler, K., Wagner, E., and Ettmayer, P. (1994) A method for the determination of the absolute-configuration of chiral alkanols. Chemische Berichte 127, 887-892.
  • Curiel, T.J., Cook, D.R., Bogedain, C., Jilg, W., Harrison, G.S., Cotten, M., Curiel, D.T., and Wagner, E. (1994) Foreign gene expression in Epstein-Barr virus transformed human B cells, Virology 198, 577-585.
  • Plank, C., Oberhauser, B., Mechtler, K., Koch, C., and Wagner, E. (1994) The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems. J. Biol. Chem. 269, 12918-12924.
  • Wagner, E., Cotten, M., Plank, C., Mechtler, K., Zatloukal, K., and Birnstiel, M.L. (1994) Receptor-mediated gene delivery into mammalian cells. In: "Animal cell technology: Products of today, prospects for tomorrow" R.E. Spier, J.B. Griffiths, W. Berthold (Eds.)Butterworth-Heinemann Ltd, Oxford, pp 30-34.
  • Wagner, E., Curiel, D. and Cotten, M. (1994) Delivery of drugs, proteins and genes into cells using transferrin as a ligand for receptor-mediated endocytosis. Adv. Drug Del. Rev. 14, 113-136.
  • Cheng, Q., Cant, C.A., Moll, T., Hofer-Warbinek, R., Wagner, E., Birnstiel, M.L., Bach, F.H., and de Martin, R. (1994) NF-kB Subunit-specific regulation of the IkB promoter. J. Biol. Chem . 269, 13551-13557.
  • Zatloukal, K., Cotten, M., Berger, M., Schmidt, W., Wagner, E. and Birnstiel, M.L. (1994) In vivo production of human factor VIII in mice after intrasplenic implantation of primary fibroblasts transfected by receptor-mediated, adenovirus-augmented gene delivery. Proc. Natl. Acad. Sci. USA 91, 5148-5152.
  • Wagner, E., Buschle, M., Cotten, M., Plank, C., Zauner, W., and Birnstiel, M.L. (1994) Synthetic virus-like gene delivery systems. Proc Int Symp. Control. Rel. Bioact. Mater. 21, 3-4.
  • Thurnher, M., Wagner, E., Clausen, H., Mechtler, K., Rusconi, S., Dinter, A., Berger, E.G., Birnstiel, M.L., and Cotten, M. (1994) Carbohydrate receptor-mediated gene transfer to human T-leukemic cells, Glycobiology 4, 429-435.
  • Cotten, M., Baker, A., Saltik, M., Wagner, E., and Buschle, M. (1994) Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus. Gene Therapy 1, 239-246.
  • Zelphati, O., Wagner, E., and Leserman, L. (1994) Synthesis and anti-HIV activity of thiocholesteryl-coupled phosphodiester antisense oligonucleotides incorporated into immunoliposomes. Antiviral Research 25, 13-25.
  • Maass, G., Zatloukal, K., Schmidt, W., Berger, M., Cotten, M., Buschle, M., Wagner, E., Birnstiel, M.L. (1994) Generation of tumor vaccines by adenovirus-enhanced transfection of cytokine genes into tumor cells. Nato ASI Series H88, 467-479.
  • Batra, R.K., Wang-Johanning, F., Wagner, E., Garver, R.I., Curiel, D.T. (1994) Receptor-mediated gene delivery employing lectin-binding specificity. Gene Therapy 1, 255-260.
  • Cotten, M., Saltik, M., Kursa, M., Wagner, E., Maass, G., and Birnstiel, M.L. (1994) Psoralen treatment of adenovirus particles eliminates virus replication and transcription while maintaining the endosomolytic activity of the virus capsid. Virology 205, 254-261.
  • Frank, S., Krasznai, K., Durovic, S., Lobentanz, E.-M., Dieplinger, H., Wagner, E., Zatloukal, K., Cotten, M., Utermann, G., Kostner, G. M., and Zechner, R. (1994) High-Level Expression of Various Apolipoprotein(a) Isoforms by “Transferrinfection”: The Role of Kringle IV Sequences in the Extracellular Association with Low-Density Lipoprotein. Biochemistry 33, 12329-12339.
  • Zatloukal, K., Schneeberger, A., Berger, M., Koszik, F., Schmidt, W., Wagner, E., Cotten, M., Buschle, M., Maass, G., Stingl, G., Birnstiel, M.L., (1994) Genetic modification of cells by receptor-mediated adenovirus-augmented gene delivery: a new approach for immunotherapy of cancer. Verh. Dtsch. Ges. Pathol. 78, 171-6.
  • Cook, D. R., Maxwell, I.H., Glode, L. M., Maxwell, F., Stevens, J. O., Purner, M. B., Wagner, E., Curiel, D. T., Curiel, T. J. (1994) Gene therapy for B-cell lymphoma in a SCID mouse model using an immunoglobulin-regulated diphtheria toxin gene delivered by a novel adenovirus-polylysine conjugate. Cancer Biother 9, 131-41.
  • Zauner, W., Blaas, D., Küchler, E., and Wagner, E. (1995) Rhinovirus mediated endosomal release of transfection complexes. J. Virol. 69, 1085-1092.
  • Moll, T., Czyz, M., Holzmüller, H., Hofer-Warbinek, R., Wagner, E., Winkler, H., Bach, F.H., and Hofer, E. (1995) Regulation of the tissue factor promoter in endothelial cells. J. Biol. Chem. 270, 3849-3857.
  • von Rüden, T., Stingl, L., Cotten, M., Wagner, E., and Zatloukal, K. (1995) Generation of high-titer retroviral vectors following receptor-mediated, adenovirus-augmented transfection. Biotechniques 18, 484-489.
  • Zatloukal, K., Schneeberger, A., Berger, M., Schmidt, W., Kosik, F., Kutil, R., Cotten, M., Wagner, E., Buschle, M., Maass, G., Payer, E., Stingl, G., Birnstiel, M.L. (1995) Elicitation of a systemic and protective anti-melanoma immune response by an IL-2 based vaccine: assessment of critical parameters. J.Immunol. 154, 3406-3419.
  • Buschle, M., Cotten, M., Kirlappos, H., Mechtler, K., Birnstiel, M.L., and Wagner, E. (1995) Receptor-mediated gene transfer into T-lymphocytes via binding of DNA/CD3 antibody particles to the CD3 T cell receptor complex. Human Gene Therapy 6, 753-761.
  • Oberhauser, B., Plank, C., and Wagner, E. (1995) Enhancing endosomal exit of nucleic acids using pH-sensitive viral fusion peptides. Chapter 16 in "Delivery strategies for antisense oligonucleotide therapeutics", S. Akhtar (Ed.), CRC Press, Inc, Florida, p. 247-268.
  • Prchla, E., Plank, C., Wagner, E., Blaas, D., and Fuchs, R. (1995) Virus-mediated release of endosomal content in vitro: Different Behaviour of adenovirus and Rhinovirus serotype 2. J. Cell Biology 131, 111-123.
  • Zauner, W., Kichler, A., Schmidt, W., Sinski, A., Wagner, E. (1996) Glycerol enhancement of ligand-polylysine/DNA transfection. Biotechniques 20, 905-913.
  • Wagner, E. (1996) Rezeptorvermittelter Gentransfer: Anwendung in der Tumorimmunotherapie? Chapter 37 in " Molekularbiologische Grundlagen in der Gastroenterologie", Beger et al. (Ed), Scientific publishing services (P) LTD, Bangalore.
  • Stingl, G., Wolff, K., Bröcker, E.-B., R. Mertelsmann, K. Wolff, S. Schreiber et al. (1996) Phase I Study to the Immunotherapy of metastatic malignant melanoma by a cancer vaccine consisting of autologous cancer cells transfected with the human IL-2 gene. Human Gene Therapy 7, 551-563.
  • Morrison, C., Wagner, E. (1996) Extrachromosomal recombination occurs efficiently in cells defective in various DNA repair systems. Nucleic Acid Res. 24, 2053-58.
  • Wagner, E., Cotten, M., and Zatloukal, K. (1996) Receptor-mediated gene delivery with synthetic virus-like particles. p 67-77, in: Targeting of Drugs 5: Strategies for Oligonucleotide and Gene Delivery in Therapy, Cape Sounion, Greece. Ed. Grigoriadis, G., Plenum Press, New York
  • Plank, C., Mechtler, K., Wagner, E., and Szoka, F. (1996) Complement activation by polylysine-DNA complexes. In: Targeting of Drugs 5: Strategies for Oligonucleotide and Gene Delivery in Therapy, Cape Sounion, Greece. Ed. Grigoriadis, G., Plenum Press, New York.
  • Kichler, A., Zauner, W., Morrison, C., and Wagner, E. (1996) Ligand-polylysine mediated gene transfer. ACS book. Chapter 12, 120-128, in "Artificial Self-Assembling Systems for Gene Delivery", Felgner, P.L., et al. (Ed.), American Chemical Society, Washington DC.
  • Wagner, E., Cotten, M., Berger, M., Schmidt, W., Zauner, W., Buschle, M., Schweighoffer, T., Maass, G., Zatloukal, K., Schreiber, S., Stingl, G., and Birnstiel, M.L. (1996) Adenovirus enhanced receptor mediated transferrinfection (AVET) applied to the generation of tumor vaccines. Chapter in OECD Biotechnology, Gene Delivery Systems, A state-of-the-Art Review, pp 153-162.
  • Plank, C., Mechtler, K., Szoka, F., and Wagner, E. (1996) Activation of the complement system by synthetic DNA complexes: A potential barrier for intravenous gene delivery. Hum. Gene Ther. 7, 1437-1446.
  • Cotten, M., Baker, A., Birnstiel, M.L., Zatloukal, K., Wagner, E. (1996) Adenovirus polylysine DNA conjugates. (1996) In Current Protocols in Human Genetics, John Wiley and Sons, Inc. New York. Pp. 12.3.1 – 12.3.33 .
  • Gagnoux-Palacios, L., Vailly, J., Durand-Clement, M., Wagner, E., Ortonne, J.P., and Meneguzzi, G. (1996) Functional re-expression of laminin-5 in laminin-gamma2-deficient human keratinocytes modifies cell morphology, motility, and adhesion. J. Biol. Chem. 271, 18437-44.
  • Bögre, L., Jonak, C., Mink, M., Meskiene, I., Traas, J., Ha, D.T.C., Swoboda, I., Plank, C., Wagner, E., Heberle-Bors, E., and Hirt, H. (1996). Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin. The Plant Cell 8, 417-428
  • Bögre, L., Zwerger, K., Meskiene, I., Binarova, P., Csizmadia, V., Plank, C., Wagner, E., Hirt, H., and Heberle-Bors, E. (1997) The cdc2Ms kinase is differently regulated in the cytoplasm and in the nucleus. Plant Physiology 113, 841-852.
  • Zauner, W. Kichler, A., Mechtler, K., Schmidt, W., and Wagner, E. (1997) Glycerol and polylysine synergize in their ability to rupture vesicular membranes and increase transferrin-polylysine mediated gene transfer. Exp. Cell Res. 232, 137-145.
  • Ebert, O., Finke, S., Salahi, A., Herrmann, M., Trojaneck, B., Lefterova, P., Wagner, E., Kircheis, R., Huhn, D., Schriever, F., and Schmidt-Wolf, I.G.H. (1997) Lymphocyte apoptosis: induction by gene transfer techniques. Gene Therapy 4, 296-302.
  • Kichler, A., Mechtler, K., Behr, J.-P., and Wagner, E. (1997) The influence of membrane-active peptides and helper lipids on lipospermine/DNA complex mediated gene transfer. Bioconjugate Chem. 8, 213-221.
  • Mechtler, K., and Wagner, E. (1997) Gene transfer mediated by Influenza virus peptides: The role of peptide sequences. New J. Chemistry 21, 105-111.
  • Coll, J.-L., Wagner, E., Combaret, V., Mechtler, K., Amstutz, H., Iacono-DiCacito, I., Simon, N., and Favrot, M.C. (1997) In vitro targeting and specific transfection of human neuroblastoma cells by chCE7 antibody-mediated gene transfer. Gene Therapy 4, 156-161.
  • Stingl, G., Brocker, E.B., Mertelsmann, R., Wolff, K., Schreiber, S., Kampgen, E., Schneeberger, A., Trcka, J., Brennscheidt, U., Veelken, H., Birnstiel, M.L., Zatloukal, K., Maass, G., Wagner, E., Buschle, M., Kempe, R., Weber, H.A., and Voigt, T. (1997) Phase I study to the immunotherapy of metastatic malignant melanoma by a cancer vaccine consisting of autologous cancer cells transfected with the human IL-2 gene. J.Mol. Medicine 75, 297-299.
  • Kircheis, R., Kichler, A., Wallner, G., Kursa, M., Ogris, M., Felzmann, T., Buchberger, M., and Wagner, E. (1997) Coupling of cell-binding ligands to polyethylenimine for targeted delivery. Gene Therapy 4, 409-418.
  • Felgner, P.L., Barenholz, Y., Behr, J.P., Cheng, S.H., Cullis, P., Huang, L., Jessee, J.A., Seymour, L., Szoka, F., Thierry, A.R., Wagner, E., and Wu, G. (1997) Nomenclature for synthetic gene delivery systems. Human Gene Therapy 8, 511-512.
  • Talsma, H., Cherng, J.-Y., Lehrmann, H., Kursa, M., Ogris, M., Hennink, W.E., Cotten, M., and Wagner, E. (1997) Stabilization of gene delivery systems by freeze-drying. Int. J. Pharm. 157, 233-238.
  • van Slooten, M.L., Koppenhagen, F.J., Bergers, J.J., Kircheis, R., Crommelin, D.J.A., Wagner, E., and Storm, G. (1998) Liposomal delivery of cytokines for anticancer vacination. In "Future Strategies for Drug Delivery with Particulate Systems", JE Diederichs and RH Müller (Eds), CRC Press.
  • Wagner, E. (1998) Polylysine-conjugate based DNA delivery. pp. 309-322, In: Self-assembling complexes for gene delivery. From laboratory to clinical trials, Kabanov, Felgner, Seymour (Eds), Wiley, England.
  • Finke, S., Trojanek, B., Lefterova, P., Csipai, M., Wagner, E., Kircheis, R., Neubauer, A., Huhn, D., Wittig, B., and Schmidt-Wolf, I.G.H. (1998) Increase of proliferation rate and enhancement of antitumor cytotoxicity of expanded human CD3+CD56+ immunological effector cells by receptor-mediated transfection with the IL-7 gene. Gene Therapy 5, 31-39.
  • Wagner, E., Ogris, M., and Zauner, W. (1998) Polylysine-based transfection systems utilizing receptor-mediated delivery, Adv. Drug Del. Rev. 30, 97-113.
  • Wagner, E. (1998) Effects of membrane-active agents in gene delivery. J. Controlled Release 53, 155-158.
  • Harbottle, R.P., Cooper, R.G., Hart, S.L., Ladhoff, A., McKay, T., Knight, A.M., Wagner, E., Miller, A.D., and Coutelle, C.(1998) An RGD-oligolysine peptide: a prototype construct for integrin-mediated gene delivery. Human Gene Therapy 9, 1037-1047.
  • Kichler, A., Zauner, W., Ogris, M., and Wagner, E (1998) Influence of the DNA complexation medium on the transfection efficiency of lipospermine/DNA particles. Gene Therapy 5, 855-860.
  • Diebold, S.S., Cotten, M., Wagner, E., and Zenke, M. (1998) Gene-modified dendritic cells by receptor-mediated transfection. Adv. Exp. Med. Biol. 451, 449-455.
  • Koppenhagen, F.J., Küpcü, Z., Wallner, G., Crommelin, D.J.A., Wagner, E., Storm, G., and Kircheis, R. (1998) Sustained cytokine delivery for anticancer vaccination: liposomes as alternative for gene-transfected tumor cells. Clin. Cancer Res. 4, 1881-1886.
  • Kircheis, R., Küpcü, Z., Wallner, G., Wagner, E. (1998) Cytokine gene-modified tumor cells for prophylactic and therapeutic vaccination: IL-2, IFN-gamma, or combination IL-2+IFN-gamma. Cytokines Cell. Mol. Therapy 4, 95-103.
  • Wagner, E. (1998) Receptor-mediated delivery of plasmid DNA. Biogenic Amines 14, 519-536.
  • Plank, C., Zauner, W., and Wagner, E. (1998) Application of membrane-active peptides for drug and gene delivery across cellular membranes. Adv. Drug Del. Rev. 34, 21-35.
  • Ogris, M., Steinlein, P., Kursa, M., Mechtler, K., Kircheis, R., and Wagner, E. (1998) The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Therapy 5, 1425-1433.
  • Wagner, E. Receptor-mediated gene transfer (1999) In: Gene Therapy, Principles and applications. Blankenstein (Ed), Birkhäuser, Basel, Switzerland.
  • Cotten, M. and Wagner, E. (1999) Receptor-mediated gene delivery strategies. The Development of Human Gene Therapy, CSHL Press, p. 261-277.
  • Wagner, E. (1999) Gentherapie, In: Pathophysiologie, Wick, et al (Eds), Gustav-Fischer-Verlag, Stuttgart.
  • Ogris, M., Brunner, S., Schueller, S., Kircheis, R. and Wagner, E. (1999) PEGylated DNA/Transferrin-PEI complexes: Reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Therapy 6, 595-605.
  • Kircheis, R., Schueller, S., Brunner, S., Ogris, M., Heider, K.-H., Zauner, W., and Wagner, E. (1999) Polycation-based DNA complexes for tumor-targeted gene delivery in vivo. J. Gene Medicine 1, 111-120.
  • Diebold, S.S., Lehrmann, H., Kursa, M., Wagner, E., Cotten, M., and Zenke, M. (1999) Efficient Gene Delivery into Human Dendritic Cells by Adenovirus Polyethylenimine and Mannose Polyethylenimine transfection. Human Gene Therapy 10, 775-786.
  • Schreiber, S., Kämpgen, E., Wagner, E., Pirkhammer, D., Trcka, J., Korschan, H., Lindemann, A., Dorffner, R., Kittler, H., Kasteliz, F., Küpcü, Z., Sinski, A., Zatloukal, K., Buschle, M., Schmidt, W., Birnstiel, M.L., Kempe, R.E., Voigt, T., Weber, H., Pehamberger, H., Mertelsmann, R., Bröcker, E.B., Wolff, K., and Stingl, G. (1999) Immunotherapy of metastatic malignant melanoma by a vaccine consisting of autologous interleukin 2-transfected cancer cells: Outcome of a Phase I Study. Human Gene Therapy 10, 983-993.
  • Zauner, W., Brunner, S., Buschle, M., Ogris, M., Wagner, E. (1999) Differential behaviour of lipid based and polycation based gene transfer systems in transfecting primary human fibroblasts: a potential role of polylysine in nuclear transport. Biophys. Biochim. Acta 1428, 57-67.
  • Wagner, E. (1999) Ligand-polycation conjugates for receptor-targeted gene transfer. Chapter 10 (p. 208-227) in "Non-Viral Vectors for Gene Therapy", L. Huang, M.C. Hung, E. Wagner (Eds), Academic Press.
  • Diebold, S.S., Kursa, M., Wagner, E., Cotten, M., and Zenke, M. (1999) Mannose-polyethylenimine (ManPEI) conjugates for targetted DNA delivery into dendritic cells. J. Biol. Chem. 274, 19087-19094.
  • van Slooten, M.L., Kircheis, R., Koppenhagen, F.J., Wagner, E., Storm, G. (1999) Liposomes as cytokine-supplement in tumor cell-based vaccines. Int. J. Pharm. 183, 33-36.
  • Wagner, E. (1999) Application of membrane-active peptides for nonviral gene delivery. Adv. Drug Del. Rev. 38, 279-289.
  • Storm, G. and Wagner, E. (1999) Volume theme title: Membrane destabilization for improved cytosolic delivery. (Theme Eds. G. Storm, E. Wagner) Preface. Adv. Drug Del. Rev. 38, 195.
  • Kircheis, R., Ogris, M., Brunner, S., Schüller, S., Wightman, L., Kursa, M. and Wagner, E. (1999) Transferrin-polycation DNA complexes for tumor-targeted in vivo gene transfer. In: Progress in Drug Delivery Systems VIII, N. Oku, Ed., 83-94.
  • Culver, K.W. and Wagner, E. (1999) An introduction to Current Opinion in Molecular Therapeutics. Curr. Opin. Mol. Ther. 1, 1-2.
  • Wightman, L., Patzelt, E., Wagner, E., and Kircheis, R. (1999) Development of transferrin-polycation/DNA based vectors for gene delivery to melanoma cells. J. Drug Targeting 7, 293-303.
  • Schober, D., Bayer, N., Murphy, R.F., Wagner, E., and Fuchs, R. (1999) Establishment of an assay to determine adenovirus-induced endosome rupture required for receptor-mediated gene delivery. Gene Ther Mol Biol. 3, 25-33.